|Description ||Heterotrophic Plate Count (Pour Plate Method)
|Method Summary||A 1 mL aliquot of sample is distributed evenly in a petri dish and combined with melted plate count agar. This analysis is done in duplicate for all samples. Both petri dishes are incubated for 48 hours at 35°C. After their respective incubation times, heterotrophic bacteria are enumerated. The two enumerations are then averaged to obtain a final result.
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|Scope and Application||The heterotrophic plate count is useful in enumerating bacteria in water and measuring changes during water treatment and distribution.
|Heterotrophic Bacteria Organism||HPC measures a range of bacteria that are naturally present in the environment. HPC has no health effects; it is an analytic method used to measure all bacteria that are common in water. The lower the concentration of bacteria in drinking water, the better maintained the water system is.
|Result Interpretation||CFU/1 mL = Colony Forming Units in 1 mL of Sample|