Microbiological Fact Sheet

Description Heterotrophic Plate Count (Pour Plate Method)
Method SM9215B
Method SummaryA 1 mL aliquot of sample is distributed evenly in a petri dish and combined with melted plate count agar. This analysis is done in duplicate for all samples. Both petri dishes are incubated for 48 hours at 35°C. After their respective incubation times, heterotrophic bacteria are enumerated. The two enumerations are then averaged to obtain a final result.

Link to the National Library of Medicine's Toxicology
Database for more references on this analyte.

Scope and ApplicationThe heterotrophic plate count is useful in enumerating bacteria in water and measuring changes during water treatment and distribution.
Heterotrophic Bacteria OrganismHPC measures a range of bacteria that are naturally present in the environment. HPC has no health effects; it is an analytic method used to measure all bacteria that are common in water. The lower the concentration of bacteria in drinking water, the better maintained the water system is.
Result InterpretationCFU/1 mL = Colony Forming Units in 1 mL of Sample

DISCLAIMER - Please Read

Florida-Spectrum List of Services
Florida-Spectrum Homepage