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<TITLE>Spectrum Environmental Testing Laboratories : Microbiological Fact Sheet SM9215B “Heterotrophic Plate Count (Pour Plate Method)”
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<TABLE BORDER><TR><TH>Description </TH><TD>Heterotrophic Plate Count (Pour Plate Method)
</TD></TR>
<TR><TH>Method</TH><TD>


SM9215B</TD></TR>

<TR><TH>Method Summary</TH><TD>A 1 mL aliquot of sample is distributed evenly in a petri dish and combined with melted plate count agar.  This analysis is done in duplicate for all samples. Both petri dishes are incubated for 48 hours at 35°C.  After their respective incubation times, heterotrophic bacteria are enumerated. The two enumerations are then averaged to obtain a final result. 
<tr><td colspan="2" align ="center"> <a href="http://toxnet.nlm.nih.gov/cgi-bin/sis/search/r?dbs+toxline:Heterotrophic+Plate"><h4>Link to the National Library of Medicine's Toxicology<br> Database for more references
on this analyte.</h4></A></TD></TR>
<TR><TH>Scope and Application</TH><TD>The heterotrophic plate count is useful in enumerating bacteria in water and measuring changes during water treatment and distribution. 
</TD></TR>
<TR><TH>Heterotrophic Bacteria Organism</TH><TD>HPC measures a range of bacteria that are naturally present in the environment. HPC has no health effects; it is an analytic method used to measure all bacteria that are common in water. The lower the concentration of bacteria in drinking water, the better maintained the water system is.
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<TR><TH>Result Interpretation</TH><TD>CFU/1 mL = Colony Forming Units in 1 mL of Sample</TD></TR>


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